Difference between revisions of "Part:BBa K2365048"
(→300px|center) |
(→300px|center) |
||
| Line 19: | Line 19: | ||
===[[File:Bax.png|300px|center]]=== | ===[[File:Bax.png|300px|center]]=== | ||
Figure1: Picture of gel electrophoresis (PCR validated products of Bax mutant form1 ; 579bp) | Figure1: Picture of gel electrophoresis (PCR validated products of Bax mutant form1 ; 579bp) | ||
| − | + | Function：We use Bax protein to kill our engineered yeast when the fungal has finished its mission. | |
| − | + | ||
| − | + | ||
===[[File:图片3.png|300px|center]]=== | ===[[File:图片3.png|300px|center]]=== | ||
Revision as of 10:52, 31 October 2017
Bax mutant form1
Coding sequence of Bax mutant form1 protein which releases cytochrome C and leads to apoptosis in the most broadly used eukaryotic chassis. This year we choose Saccharomyces cerevisiae as our chassis fungal, so we use this gene as the key gene in our biosafety device. It worked well when we detected it's function.We submitted as a improvement of already exist hbax biobrick. The link of original biobrick is here:https://parts.igem.org/Part:BBa_K364202
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 452
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Figure1: Picture of gel electrophoresis (PCR validated products of Bax mutant form1 ; 579bp) Function：We use Bax protein to kill our engineered yeast when the fungal has finished its mission.
