Difference between revisions of "Part:BBa K2387045"

 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2387045 parameters</partinfo>
 
<partinfo>BBa_K2387045 parameters</partinfo>
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In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).
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[[file:https://static.igem.org/mediawiki/2017/5/55/T--Wageningen_UR--mVenus.jpg|400px|center|thumb|<p align="justify">'''Figure 1: Absorbance and fluorescence spectra of mVenus after induction with 0.002% arabinose.'''</p>]]
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This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system ([[BBa_K2387032]]). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.
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<table>
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<tr>
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<th colspan="3">Table 1: Split Proteins.</th>
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</tr>
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<tr>
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<th>Protein</th>
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<th>Part Number (Full length)</th>
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<th>Part Number (Split Protein)</th>
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</tr>
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<tr>
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<td>mRFP</td>
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<td>[[BBa_K2387054]]</td>
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<td>[[BBa_K2387055]]</td>
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</tr>
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<tr>
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<td>eYFP</td>
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<td>[[BBa_K2387003]]</td>
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<td>[[BBa_K2387065]]</td>
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</tr>
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<tr>
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<td>mVenus</td>
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<td>[[BBa_K2387045]]</td>
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<td>[[BBa_K2387046]]</td>
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</tr>
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<tr>
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<td>sfGFP</td>
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<td>[[BBa_K2387047]]</td>
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<td>[[BBa_K2387048]]</td>
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</tr>
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<tr>
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<td>mCerulean</td>
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<td>[[BBa_K2387052]]</td>
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<td>[[BBa_K2387053]]</td>
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</tr>
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</table>

Revision as of 10:50, 31 October 2017


mVenus controlled by inducible araC/pBAD promoter

mVenus is a bright yellow monomeric fluorescent protein. In this composite, its expression is regulated by the araC/pBad promoter and by a strong RBS. mVenus presents a maximum pick of excitation at 515 nm and a maximum pick of emission at 528. mVenus was engineered from YFP to grant it a faster maturation and to make more resistant to pH and chloride ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Functional Parameters

absMax. 515 nm
chassisE. coli
colorYellow Fluorescence, Pale Yellow cells
emissionMax. 528 nm

In order to test the functionality of this protein under the araC/pBad promoter, the absorbance and the fluorescence spectra were measured after induction with 0.002% arabinose (Figure 1).

File:Https://static.igem.org/mediawiki/2017/5/55/T--Wageningen UR--mVenus.jpg

Figure 1: Absorbance and fluorescence spectra of mVenus after induction with 0.002% arabinose.

This protein is part of a collection of split proteins developed for BiFC analysis and tested with the CpxR system (BBa_K2387032). These proteins were characterized through multiple experiments using as interacting proteins antiparallel synthetic leucine zippers. The proteins of this collection are the ones found in Table 1.

Table 1: Split Proteins.
Protein Part Number (Full length) Part Number (Split Protein)
mRFP BBa_K2387054 BBa_K2387055
eYFP BBa_K2387003 BBa_K2387065
mVenus BBa_K2387045 BBa_K2387046
sfGFP BBa_K2387047 BBa_K2387048
mCerulean BBa_K2387052 BBa_K2387053