Difference between revisions of "Part:BBa K2404018"
 
Line 14: Line 14:
 
<span class='h3bb'>Sequence and Features</span><br>
 
<span class='h3bb'>Sequence and Features</span><br>
 
1-6: BsmBI<br>
 
1-6: BsmBI<br>
−
16-516: PDF1.2<br>
+
16-516: [https://parts.igem.org/Part:BBa_K2404002 PDF1.2]<br>
−
517-1329: TSHH<br>
+
517-1329: [https://parts.igem.org/Part:BBa_K2404001 TSHH]<br>
 
1334-1339: BsmBI<br>
 
1334-1339: BsmBI<br>
 
<partinfo>BBa_K2404018 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2404018 SequenceAndFeatures</partinfo>

Latest revision as of 10:49, 31 October 2017


TSH antagonist under control of the PDF1.2 promoter

This construct contains the PDF1.2 promotor, and the TSHH coding sequence. This does not contain a terminator sequence.
This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The PDF1.2 promoter is induced by the plant hormone jasmonic acid.


Sequence and Features
1-6: BsmBI
16-516: PDF1.2
517-1329: TSHH
1334-1339: BsmBI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 412
    Illegal BamHI site found at 1303
    Illegal XhoI site found at 1115
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1048
    Illegal PstI site found at 1090
  • 1000
    COMPATIBLE WITH RFC[1000]