Difference between revisions of "Part:BBa K2404016"

Latest revision as of 09:46, 31 October 2017

A TSH antagonist with His-Tags, controlled by the LexA promoter

This construct contains the LexA promotor, the TSHH coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.

Sequence and Features Sequence details:
1-6: BsmBI:
12-356: LexA
357-1169: TSHH
1174-1436: NosT
1456-1461: BsmBI

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 22
Illegal PstI site found at 888
Illegal PstI site found at 930
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 22
Illegal PstI site found at 888
Illegal PstI site found at 930
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1143
Illegal XhoI site found at 955
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 22
Illegal PstI site found at 888
Illegal PstI site found at 930
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 22
Illegal PstI site found at 888
Illegal PstI site found at 930
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2404016 short</partinfo><br>
-This construct contains the [https://parts.igem.org/Part:BBa_P10000 LexA promotor], the [[https://parts.igem.org/Part:BBa_K2404001 TSHH] coding sequence and the [https://parts.igem.org/Part:BBa_P10401 Nos terminator].
+This construct contains the [https://parts.igem.org/Part:BBa_P10000 LexA promotor], the [https://parts.igem.org/Part:BBa_K2404001 TSHH] coding sequence and the [https://parts.igem.org/Part:BBa_P10401 Nos terminator].
 This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10503 pGB-A2 plasmid].