Difference between revisions of "Part:BBa K2404016"
Line 15: Line 15:
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 +
Sequence details:
 +
<br>
 
1-6: BsmBI:<br>
 
1-6: BsmBI:<br>
−
7-348: LexA:<br>
+
12-356: LexA<br>
−
349-2005: LUC+:<br>
+
357-1169: TSHH<br>
−
2006-2272: NosT:<br>
+
1174-1436: NosT<br>
−
2282-2287: BsmBI:<br>
+
1456-1461: BsmBI<br>
 +
 
  
 
<partinfo>BBa_K2404016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2404016 SequenceAndFeatures</partinfo>

Revision as of 09:45, 31 October 2017


A TSH antagonist with His-Tags, controlled by the LexA promoter

This construct contains the LexA promotor, the [TSHH coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A2 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.


Sequence and Features Sequence details:
1-6: BsmBI:
12-356: LexA
357-1169: TSHH
1174-1436: NosT
1456-1461: BsmBI



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1143
    Illegal XhoI site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 1000
    COMPATIBLE WITH RFC[1000]