Difference between revisions of "Part:BBa K2213003"
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The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs PPK (BBa_*********) and mCherry-PPK (BBa_*********). | The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs PPK (BBa_*********) and mCherry-PPK (BBa_*********). | ||
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Revision as of 09:18, 31 October 2017
cgPPK2_His6
Cornybacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal
Usage and Biology
Characterisation
Assessing the thermal stability of cgPPK2 and cgPPK2-mCherry
The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs PPK (BBa_*********) and mCherry-PPK (BBa_*********).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 761