Difference between revisions of "Part:BBa K2404016"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2404016 short</partinfo>
 
<partinfo>BBa_K2404016 short</partinfo>
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We generated a luciferase construct to test the efficacy of the tobacco leave expression system.
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This construct contains the [https://parts.igem.org/Part:BBa_P10000 LexA promotor], the luciferase coding sequence and the [https://parts.igem.org/Part:BBa_P10401 Nos terminator].
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This part is contained within the Phytobrick level 1 [https://parts.igem.org/Part:BBa_P10501 pGB-A1 plasmid].
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<br>
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The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.
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A thyroid stimulating hormone antagonist, with His-Tags for purification, under the control of the LexA XVE-inducible promoter native to <i> E. coli </i>. This has the Nopaline synthase gene terminator from <i> Agrobacterium tumefaciens </i>. The construct is contained within the pGB-A2 plasmid. It should be used to produce the TSH antagonist under control of XVE.
 
A thyroid stimulating hormone antagonist, with His-Tags for purification, under the control of the LexA XVE-inducible promoter native to <i> E. coli </i>. This has the Nopaline synthase gene terminator from <i> Agrobacterium tumefaciens </i>. The construct is contained within the pGB-A2 plasmid. It should be used to produce the TSH antagonist under control of XVE.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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1-6: BsmBI:<br>
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7-348: LexA:<br>
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349-2005: LUC+:<br>
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2006-2272: NosT:<br>
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2282-2287: BsmBI:<br>
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<partinfo>BBa_K2404016 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2404016 SequenceAndFeatures</partinfo>
 +
  
  

Revision as of 09:07, 31 October 2017


A TSH antagonist with His-Tags, controlled by the LexA promoter We generated a luciferase construct to test the efficacy of the tobacco leave expression system.

This construct contains the LexA promotor, the luciferase coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A1 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.


A thyroid stimulating hormone antagonist, with His-Tags for purification, under the control of the LexA XVE-inducible promoter native to E. coli . This has the Nopaline synthase gene terminator from Agrobacterium tumefaciens . The construct is contained within the pGB-A2 plasmid. It should be used to produce the TSH antagonist under control of XVE.

Sequence and Features 1-6: BsmBI:
7-348: LexA:
349-2005: LUC+:
2006-2272: NosT:
2282-2287: BsmBI:


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1143
    Illegal XhoI site found at 955
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
    Illegal PstI site found at 888
    Illegal PstI site found at 930
  • 1000
    COMPATIBLE WITH RFC[1000]