Difference between revisions of "Part:BBa K2404014"
 
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<partinfo>BBa_K2404014 short</partinfo>
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<partinfo>BBa_K2404014 short</partinfo><br>
 
We generated a luciferase construct to test the efficacy of the tobacco leave expression system.  
 
We generated a luciferase construct to test the efficacy of the tobacco leave expression system.  
  

Latest revision as of 08:54, 31 October 2017


Luc+ gene under control of the LexA promoter
We generated a luciferase construct to test the efficacy of the tobacco leave expression system.

This construct contains the LexA promotor, the luciferase coding sequence and the Nos terminator.

This part is contained within the Phytobrick level 1 pGB-A1 plasmid.
The LexA promoter is induced by the XVE protein in the presence of estradiol so to be active requires co-expression with the XVE protein.

Sequence and Features Sequence detail:
:
1-6: BsmBI:
7-348: LexA:
349-2005: LUC+:
2006-2272: NosT:
2282-2287: BsmBI:


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1171
    Illegal BamHI site found at 1017
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 15
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]