Difference between revisions of "Part:BBa K354001"

 
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K354001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K354001 SequenceAndFeatures</partinfo>
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===Improvement:TMMU-China 2017===
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Author：Yizhen Xu
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Summary：In this improvement, we splited this part into two parts that can be used in cell-cell communication.
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From reference [1], the PlcR-PapR fusion peptide can activate gene expression without the PapR signal peptide. Because alteration of the export signal, this fusion peptide can not be exported and re-imported into the cell to activate the PlcR transcription factor. In our improvement, we provided two new parts. One is the PlcR protein (BBa_K2279002), the other is PapR signal peptide (BBa_K2279003). Combining these two new parts, we can build autoinduction system and Sender-Receiver Cells to enable cell-cell communication.
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===Reference===
  
  

Revision as of 08:20, 31 October 2017


PlcR-PapR - Gram+ve Quorum Peptide

The PlcR-PapR fusion peptide has been derived from the Bacillus cereus quorum signalling system. This group of Gram-positive bacteria employs the PlcR quorum system to initiate virulence factors under appropriate cell density conditions. Synthesized as a propeptide, this fusion molecule is exported from the cell via the secA pathway, cleaved by an extracellular protease and re-imported into the cell via an oligopeptide permease. Both the extracellular protease as well as olidopeptide permease are ubiquitous structures to the Gram-positive cell wall. A such, any gram-positive bacteria expressing the PlcR-PapR protein should be able to both process as well as import the activating form of the fusion peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Improvement:TMMU-China 2017

Author：Yizhen Xu Summary：In this improvement, we splited this part into two parts that can be used in cell-cell communication.

From reference [1], the PlcR-PapR fusion peptide can activate gene expression without the PapR signal peptide. Because alteration of the export signal, this fusion peptide can not be exported and re-imported into the cell to activate the PlcR transcription factor. In our improvement, we provided two new parts. One is the PlcR protein (BBa_K2279002), the other is PapR signal peptide (BBa_K2279003). Combining these two new parts, we can build autoinduction system and Sender-Receiver Cells to enable cell-cell communication.

Reference