Difference between revisions of "Part:BBa K2311001"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2311001 short</partinfo>
 
<partinfo>BBa_K2311001 short</partinfo>
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This part has a positive feedback loop, so the expression of GFP can enhance quickly. The backgroud expression could automatically open the system by itself. Adding AHL could change the openning time. But this system require more time to show positive feedback effect than no AiiA controlling.
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This part has a positive feedback loop, so the expression of GFP can enhance quickly. In General, a positive feedback loop can be triggered by a low input such as PCR. This positive feedback loop will be opened by background expression of pLuxR in E.coli. But different input lead to different opening time.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:14, 31 October 2017


GFP producer controlled by 3OC6-HSL This part has a positive feedback loop, so the expression of GFP can enhance quickly. The backgroud expression could automatically open the system by itself. Adding AHL could change the openning time. But this system require more time to show positive feedback effect than no AiiA controlling.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2513
    Illegal NheI site found at 2536
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2501
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2282