Difference between revisions of "Part:BBa K2371004"
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[[File:BGIC-Union Part sgRNA Figure1 plasmid.png|700px]]
 
[[File:BGIC-Union Part sgRNA Figure1 plasmid.png|700px]]
  
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Figure 1. The plasmid of sgRNA generator. The sgRNA generator Biobrick is composed of a T7 promotor, coding sequence for sgRNA and a T7 terminator
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<h1>Figure 1. The plasmid of sgRNA generator. The sgRNA generator Biobrick is composed of a T7 promotor, coding sequence for sgRNA and a T7 terminator.<h1>
  
  

Revision as of 07:03, 31 October 2017


sgRNA generator for EML4-ALK variant A 23 This specific sgRNA generator is for detection of EML4-ALK cancer gene detection in pair with BGIC-Union NT7-dCas9(BBa_K2371000) and CT7-dCas9(BBa_K237101). This part is designed with T7 promotor and T7 terminator and can effectively generate specific RNA in vitro. The product can have RNA concentration of 1500 ng/ul or higher with MEGAscript™ T7 Transcription Kit and after the purification and collection using Ambion® mirVana™ miRNA Isolation Kit. BGIC-Union Part sgRNA Figure1 plasmid.png

Figure 1. The plasmid of sgRNA generator. The sgRNA generator Biobrick is composed of a T7 promotor, coding sequence for sgRNA and a T7 terminator.<h1> Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]