Difference between revisions of "Part:BBa K2371004"
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<partinfo>BBa_K2371004 short</partinfo>
 
<partinfo>BBa_K2371004 short</partinfo>
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This specific sgRNA generator is for detection of EML4-ALK cancer gene detection in pair with BGIC-Union NT7-dCas9(BBa_K2371000) and CT7-dCas9(BBa_K237101). This part is designed with T7 promotor and T7 terminator and can effectively generate specific RNA in vitro. The product can have RNA concentration of 1500 ng/ul or higher with MEGAscript™ T7 Transcription Kit and after the purification and collection using Ambion® mirVana™ miRNA Isolation Kit.
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[[File:https://parts.igem.org/File:BGIC-Union_Part_sgRNA_Figure1_plasmid.png]]
  
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This specific sgRNA generator is for detection of EML4-ALK cancer gene detection in pair with BGIC-Union NT7-dCas9(BBa_K2371000) and CT7-dCas9(BBa_K2371001).
 
  
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[[File:http://https://static.igem.org/mediawiki/parts/0/07/%E6%8D%95%E8%8E%B7.PNG.jpg]]
 
  
 
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Revision as of 06:52, 31 October 2017


sgRNA generator for EML4-ALK variant A 23 This specific sgRNA generator is for detection of EML4-ALK cancer gene detection in pair with BGIC-Union NT7-dCas9(BBa_K2371000) and CT7-dCas9(BBa_K237101). This part is designed with T7 promotor and T7 terminator and can effectively generate specific RNA in vitro. The product can have RNA concentration of 1500 ng/ul or higher with MEGAscript™ T7 Transcription Kit and after the purification and collection using Ambion® mirVana™ miRNA Isolation Kit. File:Https://parts.igem.org/File:BGIC-Union Part sgRNA Figure1 plasmid.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]