Difference between revisions of "Part:BBa K2295003"
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[[Image:T-FREIBURG-HIF1A_shRNA2_134-50pc.png|900px|thumb|center|
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'''Figure 1: Flow cytometry of hypoxia response element promoter tests. '''
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<p><b>a)</b> Jurkat, <b>b)</b> HEK293T cells stably transduced with 4xHRE-pTal:eCFP, and <b>c)</b> HRE-pTal:eCFP or <b>d)</b> 4xHRE-pTal:eCFP PEI transfected into CHO-K1 cells. For analysis cells were incubated 24 h with indicated concentrations of CoCl<sub>2</sub>. Significant differences were determined using one-tailed student’s t-test (Excel 2017) followed by Bonferroni-Hoch correction; * p ** p non-significant and decreasing differences are not marked.</p>]]
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 03:29, 31 October 2017


hre (hypoxia response element) ptal (minimalpromoter)


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Figure 1: Flow cytometry of hypoxia response element promoter tests.

a) Jurkat, b) HEK293T cells stably transduced with 4xHRE-pTal:eCFP, and c) HRE-pTal:eCFP or d) 4xHRE-pTal:eCFP PEI transfected into CHO-K1 cells. For analysis cells were incubated 24 h with indicated concentrations of CoCl2. Significant differences were determined using one-tailed student’s t-test (Excel 2017) followed by Bonferroni-Hoch correction; * p ** p non-significant and decreasing differences are not marked.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 51
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

https://parts.igem.org/Part:BBa_K1456004