Difference between revisions of "Part:BBa K2295002"

Revision as of 03:10, 31 October 2017

HIF1A (Hypoxia-inducible factor one alpha)

Overview

Western Blot against HIF1A

Figure 7: Transient introduction of artificial HIF1A in HIF1A shRNA2 knockdown HEK293T cell line.

Induction of the overexpressed HIF1A-HA can be observed in the last lane. Endogenous and artificial HIF1A were detected with HIF1A antibody; reintroduced HIF1A was detected via an HA-tag; GAPDH served as a loading control. Wild type (WT) and knockdown (HIF1A shRNA2) cells were transfected with CMV:HIF1A-HA one day prior to induction with CoCl₂ to simulate hypoxia. Cells were harvested 24 hours after induction. Immunoblot assay was performed using antibodies against HIF1A, HA-tag and GAPDH.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 79
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 10
Illegal AgeI site found at 1086
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2295002 short</partinfo>
-Hif1a
+<h2>Overview</h2>
+<h2>Western Blot against HIF1A</h2>
 [[Image:T-FREIBURG-HIF1A_shRNA2_134-50pc.png|900px|thumb|center|'''Figure 7: Transient introduction of artificial HIF1A in HIF1A shRNA2 knockdown HEK293T cell line.'''<p>
                         Induction of the overexpressed HIF1A-HA can be observed in the last lane. Endogenous and artificial HIF1A were detected with HIF1A antibody; reintroduced HIF1A was detected via an HA-tag; GAPDH served as a loading control. Wild type (WT) and knockdown (HIF1A shRNA2) cells were transfected with CMV:HIF1A-HA one day prior to induction with CoCl<sub>2</sub> to simulate hypoxia. Cells were harvested 24 hours after induction. Immunoblot assay was performed using antibodies against HIF1A, HA-tag and GAPDH.</p>]]