Difference between revisions of "Part:BBa I2032"

 
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Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP).  This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to <i>E. coli</i> RNAP.
 
Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP).  This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to <i>E. coli</i> RNAP.
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<h1> Improved Part </h1>
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<p><b>Improved part:</b> BBa_K2443037 submitted by Lethbridge iGEM 2017</p>
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<p><b> Rational behind improvements:</b>  BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next vivo system we have Codon optimized for use in E. coli and attached a N-terminal hexahistidine tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21-Gold (DE3) cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015). </p>
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Revision as of 01:24, 31 October 2017


T7 RNA polymerase

Gene 1 coding region from bacteriophage T7 encoding a RNAP polymerase (RNAP). This single-protein RNAP recognizes a 21 bp consensus promoter sequence and is orthogonal to E. coli RNAP.

Improved Part

Improved part: BBa_K2443037 submitted by Lethbridge iGEM 2017

Rational behind improvements: BBa_I2032 encodes exclusively for the coding region of T7 RNA polymerase. In order to improve it and incorporate it into our Next vivo system we have Codon optimized for use in E. coli and attached a N-terminal hexahistidine tag with a serine glycine linker for easy purification. We have also optimized our T7 RNA polymerase to be overexpressed in BL21-Gold (DE3) cells by putting it under the control of a T7 promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]