Difference between revisions of "Part:BBa K2440019"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker. | ||
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| + | Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.1 | ||
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| + | By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells2. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 01:11, 31 October 2017
miR-2467 target sequence
It is the target sequence of miR-2467, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker.
Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.1
By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells2.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.