Difference between revisions of "Part:BBa K2440017"

Revision as of 01:05, 31 October 2017

miR-875 target sequence

It is the target sequence of miR-875, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker.

Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.1

In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. The expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.2

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

@@ Line 8: / Line 8: @@
 MiRNA locker assembled by using this modularized DNA part was able to bind miR-875 in an Ago2 dependent manner, that is, knockdown of miR-875 was achieved by transfecting cells with miRNA locker.
+Hsa-miR-875-3p was found in human cells, and it is located in rn3 chr7- 71209979-71209999.1
+In dilated cardiomyopathy patients, the expression levels of miR-618, miR-875-3p, miR-205, miR-194, miR-302a, miR-147, and miR-544 were found decreased. The expression levels of miR-518f and miR-454 were found increased in DCM patients. These miRNA level differences may provide the chance of using these miRNAs as new biomarkers.2
 ===Sequence and Features===