Difference between revisions of "Part:BBa K2213003"
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Revision as of 22:07, 30 October 2017


cgPPK2_His6

Cornybacterium PPK2 with a C terminal polyhistidine tag, and the PduD(1-20) localisation tag at the N terminal


Usage and Biology



https://static.igem.org/mediawiki/parts/f/f8/Manchesterigem17-thermalshiftassay-ppk-mcherryppk-graph1.tiff

Characterisation


Assessing the thermal stability of cgPPK2 and cgPPK2-mCherry
The heat stability of these constructs was determined through the use of a Thermal Shift Assay of the constructs PPK (BBa_*********) and mCherry-PPK (BBa_*********).

Manchesterigem17-thermalshiftassay-ppk-mcherryppk-graph1.tiff
https://static.igem.org/mediawiki/parts/f/f8/Manchesterigem17-thermalshiftassay-ppk-mcherryppk-graph1.tiff

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 82
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 761