Difference between revisions of "Part:BBa K2380007"
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Due to the fact that we brought up the Part BBa_K2380027 in this part, you can change the Promoter with the RBS if there is the need of another one.
 
Due to the fact that we brought up the Part BBa_K2380027 in this part, you can change the Promoter with the RBS if there is the need of another one.
  
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<h2>Plasmidcard</h2>
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https://static.igem.org/mediawiki/parts/5/5d/T--TU_Darmstadt--chiAAraC-r.png
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===Usage and Biology===
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Revision as of 21:18, 30 October 2017


Arabinose inducible Chitinase A1

We combined the ChiA gene for the Chitinase A1 with the Arabinose inducible Promoter araC-Pbad (Part:BBa_K808000). This opens the opportunity to determine the length of Chitosan to dimers if it is necessary. Due to the fact that we brought up the Part BBa_K2380027 in this part, you can change the Promoter with the RBS if there is the need of another one.

Plasmidcard

T--TU_Darmstadt--chiAAraC-r.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1390
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1496
    Illegal AgeI site found at 2192
    Illegal AgeI site found at 2480
    Illegal AgeI site found at 2543
    Illegal AgeI site found at 2606
    Illegal AgeI site found at 2936
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961