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Revision as of 21:05, 30 October 2017


Chitin synthase NodC with RBS and Anderson-Promoter

The Chitin Synthase (CHS) NodC from Rhizobium leguminosarum is a N-acetylglucosaminyl transferase which catalyzes the formation of chitin pentamers by using UDP-acetylglucosamine as donor and N-acetylglucosamine as acceptor. The expression of NodC is regulated by the constitutive Anderson-promoter system (BBa_K2380025).

Plasmidcard

T--TU_Darmstadt--nodCAnderson-r.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 36
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 438
  • 1000
    COMPATIBLE WITH RFC[1000]