Difference between revisions of "Part:BBa K2404001:Design"
(→‎Design Notes)
Line 11: Line 11:
  
  
−
- We ordered the following TSH gBlock from IDT
+
- We ordered the following TSH gBlock from IDT<br>
 
https://static.igem.org/mediawiki/parts/2/2d/T--Cardiff_Wales--TSH_gblock.png
 
https://static.igem.org/mediawiki/parts/2/2d/T--Cardiff_Wales--TSH_gblock.png
  

Revision as of 20:49, 30 October 2017


A Thyroid Stimulating Hormone antagonist with His-Tags


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 795
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 827


Design Notes

We generated this part through the following steps:


- We ordered the following TSH gBlock from IDT
T--Cardiff_Wales--TSH_gblock.png

- This was introduced into pSB1C3 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning

Source

This modified protein was first used in [http://www.jbc.org/content/276/7/4543.long Fares et al (2001) JBC 276, 4543-4558] but without the His-tags. It was ordered as a g-block.

References