Difference between revisions of "Part:BBa K2323004"
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Uses a T7 promoter and RBS B0034 (BBa_K525998) to express Lwa Cas13a (BBa_K2323000) flanked by a 6x His-Tag to allow purification and a SUMO-tag to improve solubility. This is terminated by a Tphi terminator. The whole coding sequence is also available as BBa_K2323002.  
 
Uses a T7 promoter and RBS B0034 (BBa_K525998) to express Lwa Cas13a (BBa_K2323000) flanked by a 6x His-Tag to allow purification and a SUMO-tag to improve solubility. This is terminated by a Tphi terminator. The whole coding sequence is also available as BBa_K2323002.  
  
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===Usage and Biology===
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Cas13a from <i>Leptotrichia wadei</i>
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<partinfo>BBa_K2323004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2323004 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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Cas13a from <i>Leptotrichia wadei</i> (Lwa)
  
 
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Revision as of 20:23, 30 October 2017


Lwa Cas13a under T7 promoter with solubility tag

Uses a T7 promoter and RBS B0034 (BBa_K525998) to express Lwa Cas13a (BBa_K2323000) flanked by a 6x His-Tag to allow purification and a SUMO-tag to improve solubility. This is terminated by a Tphi terminator. The whole coding sequence is also available as BBa_K2323002.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1768
    Illegal BglII site found at 3448
    Illegal BamHI site found at 144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2847


Usage and Biology

Cas13a from Leptotrichia wadei (Lwa)