Difference between revisions of "Part:BBa K2262013"

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<h1>'''Introduction'''</h1>
 
<h1>'''Introduction'''</h1>
  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
It has been known as a antimicrobial peptide. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function.
 
It has been known as a antimicrobial peptide. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function.
  
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<p style="padding:1px;font-size:14px"><b>(1) Inhibition Zone </b></p>
 
<p style="padding:1px;font-size:14px"><b>(1) Inhibition Zone </b></p>
  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
  
 
<p style="padding:1px;font-size:14px"><b>(2) Spore Germination </b></p>
 
<p style="padding:1px;font-size:14px"><b>(2) Spore Germination </b></p>
  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
  
 
<p style="padding:1px;font-size:14px"><b>(3) Botany Experiment </b></p>
 
<p style="padding:1px;font-size:14px"><b>(3) Botany Experiment </b></p>
  
<p style="padding:1px;font-size:16px"><b>2. Cloning </b></p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
  
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<p style="padding:1px;font-size:16px"><b>2. Cloning </b></p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
 
We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..
 
We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..
  
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<br>
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[[File:Agarose gel Pe-4.png|200px|thumb|center|'''Figure 5.''' P<sub>T7</sub> + RBS + Pe-4 <br>
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The DNA sequence length of P<sub>T7</sub> + RBS + Pe-4 is around 100 ~ 200 b.p.. The size of its PCR product should be close to 450 ~ 500 b.p..]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:57, 30 October 2017


T7 Promoter+RBS+Pe-4

Figure 1. PT7+RBS+Pe-4+terminator

Introduction

      It has been known as a antimicrobial peptide. Its grade given by scoring card ,which was created by NCTU_Formosa, shows it may also has anti-fungal function.

Experiment

1. Fungal Test


(1) Inhibition Zone

     

(2) Spore Germination

     

(3) Botany Experiment

     


2. Cloning

      We put T7 promoter, RBS, and Pe-4 together with pSB1C3 as a backbone. Then we conducted Taq PCR to check the size of the DNA sequence was right. The length of T7 promoter+RBS+Sa-AFP2 is around 100 ~ 200 b.p.. Its PCR product is around 250 ~ 500 b.p..


Figure 5. PT7 + RBS + Pe-4
The DNA sequence length of PT7 + RBS + Pe-4 is around 100 ~ 200 b.p.. The size of its PCR product should be close to 450 ~ 500 b.p..

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]