Difference between revisions of "Part:BBa K2286005"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2286005 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2286005 SequenceAndFeatures</partinfo> | ||
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| + | ==Characterization== | ||
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| + | To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR. The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter. | ||
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| + | [[File:T--UESTC-China--PYK10-1457-RT-PCR.png|500px|thumb|center|'''Fig 1'''.RT-PCR of CKX3 activated by PYK10-1457.]] | ||
Revision as of 18:13, 30 October 2017
PYK10-1457：a seedling and root specific promoter
This part contains a root-specific promoter from Arabidopsis thaliana root and Hypocotyls. Compared to the full-length promoter, PYK10-1457 lacks the 785 bp base at the 5 'end , but its strength is increased by several times
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
To confirm whether the PPYK10-1457 promoter was strictly root-specific, we tested the roots and leaves of positive plants with RT-PCR. The results showed that the target gene CKX3 mainly express in the roots, only small amount of expression in leaves, indicating that PYK10-1457 is an excellent root-specific promoter.
