Difference between revisions of "Part:BBa K2324011"

Revision as of 17:37, 30 October 2017

T7_FimH_225sfGFP

This part synthesises a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence is under the control of an IPTG-inducible, T7 promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 451
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2324011 short</partinfo>
-This part synthesises a FimH adhesin protein fused with GFP at its 225th amino acid residue. The coding sequence is under the control of an IPTG-inducible, T7 promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .
+This part synthesises a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence is under the control of an IPTG-inducible, T7 promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the <i>fim operon</i> .
 <!-- Add more about the biology of this part here