Difference between revisions of "Part:BBa K2324006"

 
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<partinfo>BBa_K2324006 short</partinfo>
 
<partinfo>BBa_K2324006 short</partinfo>
  
This part synthesises a FimH adhesin protein fused with GFP at its 225th amino acid residue. The coding sequence
+
This part synthesises a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence
 
is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced,
 
is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced,
 
produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid
 
produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid

Revision as of 17:34, 30 October 2017


pRha_FimH_225sfGFP

This part synthesises a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should involve itself in pilus biosynthesis when co-transformed with a plasmid containing the fim operon .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]