Difference between revisions of "Part:BBa K2505002"
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This gene is derived from <i>A.thaliana</i> and optimized for <i>H.sapiens</i> codon in reference to the codon usage. AtIPT4 is an enzyme(adenylate dimethylallyltransferase '''[EC:2.5.1.112]''': cytokinin synthase) that necessary for a synthesis of iP(isopentenyl adenine: kind of cytokinin) precursor.  
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<p>This gene is derived from <i>A.thaliana</i> and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones) 
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in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112:
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cytokinin synthase) activity and catalyzes the transfer of an 
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isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP, 
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producing cytokinin nucleotides. Note that cytokinin nucleotides are the 
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immature form.</p>
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<p>The DNA sequences of these genes are optimized for expressing in human cells considering the codon usage. 
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</p>
  
 
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Revision as of 17:05, 30 October 2017

atipt4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 881


This gene is derived from A.thaliana and encodes a enzyme necessary for synthesizing precursor of isopentenyladenine (iP), a kind of cytokinin. Cytokinins are signaling molecules (Phytohormones) in plants and play important roles in cell growth and differentiation. AtIPT4 has the adenylate dimethylallyltransferase (EC:2.5.1.112: cytokinin synthase) activity and catalyzes the transfer of an isopentenyl group from dimethylallyl diphosphate (DMAPP) to ATP and ADP, producing cytokinin nucleotides. Note that cytokinin nucleotides are the immature form.

The DNA sequences of these genes are optimized for expressing in human cells considering the codon usage.


Characterization

We tested the expression of AtIPT4 protein in human cell(EA.hy626) that necessary for a synthesis of iP(isopentenyl adenine: kind of cytokinin).