Difference between revisions of "Part:BBa K2324011:Design"

 
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Revision as of 15:35, 30 October 2017


T7_FimH_225sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 451
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
Usage and Biology


Design Notes

Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.


Source

The CDS is the new part BBa_K2324001.The RBS and terminator are the registry parts BBa_B0034 and BBa_B0015 respectively. The T7 promoter is part BBa_K1614000.

References