Difference between revisions of "Part:BBa K2207011"
Doctorchen (Talk | contribs) |
|||
| Line 4: | Line 4: | ||
BBa_K2207011 | BBa_K2207011 | ||
| + | |||
generator(phlF regulated) | generator(phlF regulated) | ||
| + | |||
This part is an inducible generator regulated by PhlF. | This part is an inducible generator regulated by PhlF. | ||
It contains an inducible promoter K2207010 and a biobrick E1010. | It contains an inducible promoter K2207010 and a biobrick E1010. | ||
In the absence of PhlF protein, this part can turn the yeast Saccharomyces cerevisiaea red. | In the absence of PhlF protein, this part can turn the yeast Saccharomyces cerevisiaea red. | ||
| + | |||
In the presence of PhlF protein, this part fails to turn the yeast Saccharomyces cerevisiaea red. | In the presence of PhlF protein, this part fails to turn the yeast Saccharomyces cerevisiaea red. | ||
| + | |||
PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG). | PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG). | ||
| + | |||
PhlF can be coded by BBa_K2207009. | PhlF can be coded by BBa_K2207009. | ||
Latest revision as of 14:50, 30 October 2017
phlO-mRFP
BBa_K2207011
generator(phlF regulated)
This part is an inducible generator regulated by PhlF. It contains an inducible promoter K2207010 and a biobrick E1010.
In the absence of PhlF protein, this part can turn the yeast Saccharomyces cerevisiaea red.
In the presence of PhlF protein, this part fails to turn the yeast Saccharomyces cerevisiaea red.
PhlF can be inhibited by 2,4-diacetylphloroglucinol (DAPG).
PhlF can be coded by BBa_K2207009.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 807
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1384
Illegal AgeI site found at 1496 - 1000COMPATIBLE WITH RFC[1000]