Difference between revisions of "Part:BBa J54025:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J54025 short</partinfo> | <partinfo>BBa_J54025 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | As Suffix | + | As Suffix of Tokyo Standard. |
| + | |||
| + | *[[Tokyo Standard assembly]] | ||
| + | Tokyo Standard consists of the chain of restriction sites : | ||
| + | SalI-BamHI-part-BglII-MluI | ||
| + | |||
| + | |||
| + | BamHI sequence is "'''G^GATCC'''", and BglII sequence is "'''A^GATCT'''". | ||
| + | |||
| + | They have the sequence "'''GATC'''" in common, so you can assemble parts just like Biobrick assembly. | ||
| + | |||
Revision as of 17:07, 25 October 2007
SuffixI_BglII,MluI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As Suffix of Tokyo Standard.
Tokyo Standard consists of the chain of restriction sites : SalI-BamHI-part-BglII-MluI
BamHI sequence is "G^GATCC", and BglII sequence is "A^GATCT".
They have the sequence "GATC" in common, so you can assemble parts just like Biobrick assembly.
Source
DNA synthesis