Difference between revisions of "Part:BBa K2323005"

Latest revision as of 13:41, 30 October 2017

GFP-ASV

This GFP (E0040) is tagged with a protein degradation tag (pdt) called ASV. This causes the protein to degrade in E.coli with a first-order rate of 0.0095 per min, from the ClpP machinery of E.coli. It is under the control of a pTet promoter (aTc inducible) and the RBS is B0034. The terminator is B0015.

This biobrick is part of a collection of degradation tags, characterized on the page BBa_K2323003. See also BBa_K2323006, BBa_K2323007, BBa_K2323008.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 724

Plasmid composition

The collection is cloned from BBa_I13522 with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. (read-only benchling map) Cloning was confirmed with sequencing.

@@ Line 15: / Line 15: @@
 <partinfo>BBa_K2323005 SequenceAndFeatures</partinfo>
 ===Plasmid composition===
-[[File:BBa_K2323005.png| frame | 400px | center | The collection is cloned from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. Primers are indicated with the map ([https://benchling.com/s/w5fbeMzQ read-only benchling map]). Cloning was confirmed with sequencing. ]]
+[[File:BBa_K2323005.png| frame | 400px | center | The collection is cloned from [https://parts.igem.org/Part:BBa_I13522 BBa_I13522] with overhang PCR using 5'-phosphated primers that contain the tag as an overhang. ([https://benchling.com/s/w5fbeMzQ read-only benchling map]) Cloning was confirmed with sequencing. ]]
 <!-- Uncomment this to enable Functional Parameter display