Difference between revisions of "Part:BBa K2315011"

Revision as of 13:36, 30 October 2017

LasR-GFP

Group: Shanghaitech iGEM 2017

HSL receiver from P.aeruginosa, actives expression of GFP protein in response to 3OC12HSL.

This part is one of the interchangeable AHL receivers. Full collection could be viewed at:

Introduction

(Figure1-mechanism to be added)

When proper AHL added with concentration higher than a critical value, The constitutively expressed LasR will bind the signaling AHL molecule, dimerize and bind to pLas regulatory sequence before GFP gene and actives its expression.

Fluorescent Response to cognate 3OC12-HSL

To validate the part's design, we've used standard 3OC12HSL (HSL produced by LasI in P.aeruginosa) sample to determine the response curve of this part.

(Figure2&3-response to 3OC12HSL to be added)

Orthogonality test against non-cognate inducers

We've characterized the crosstalk between LasR and non-cognate AHLs (Figure4-7-response to non-cognate inducers to be added)

Usage: Measuring unknown AHL production

We developed a measurement protocol using the fluorescent protein coupled AHL receiver germs to measure the actual AHLs concentration in high precision and sensitivity in compared with LC-MS.

LasR-pLas-GFP Sequence and Features