Difference between revisions of "Part:BBa K2323001"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K2323001 short</partinfo>
 
<partinfo>BBa_K2323001 short</partinfo>
  
−
Uses a 6x His-Tag (BBa_K1223006) to allow purification and a SUMO-tag to improve solubility and as a cleavable tag of Lwa Cas13a (BBa_K2323000) for in-vitro use.
+
Uses a 6xHis/Twin Strep ([https://parts.igem.org/Part:BBa_K2323011 BBa_K2323011]) to allow purification and a SUMO-tag to improve solubility ([https://parts.igem.org/Part:BBa_K2323012 BBa_K2323012]) and as a cleavable tag of Lwa Cas13a (([https://parts.igem.org/Part:BBa_K2323000 BBa_K2323000])) for in-vitro use.
 +
 
 +
<!-- -->
 +
For characterization, see the full construct: [https://parts.igem.org/Part:BBa_K2323004 BBa_K2323004].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 12: Line 15:
 
<partinfo>BBa_K2323001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2323001 SequenceAndFeatures</partinfo>
  
 +
===Plasmid composition===
 +
[[File:BBa_K2323001.png| frame | 400px | center | The Biobrick [https://parts.igem.org/Part:BBa_K2323000 BBa_K2323000] is preceded by a 6xHis/Twin Strep and a SUMO tag.([https://benchling.com/s/seq-IOQUO8DFXEkAxXa8R7Op read-only benchling map]) ]]
 +
The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 12:54, 30 October 2017


Leptotrichia wadei Cas13a with a His-Tag and a SUMO-tag for protein purification.

Uses a 6xHis/Twin Strep (BBa_K2323011) to allow purification and a SUMO-tag to improve solubility (BBa_K2323012) and as a cleavable tag of Lwa Cas13a ((BBa_K2323000)) for in-vitro use.

For characterization, see the full construct: BBa_K2323004.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1736
    Illegal BglII site found at 3416
    Illegal BamHI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2815

Plasmid composition

The Biobrick BBa_K2323000 is preceded by a 6xHis/Twin Strep and a SUMO tag.(read-only benchling map)

The sequence is taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)