Difference between revisions of "Part:BBa K2323011:Design"

 
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===Design Notes===
 
===Design Notes===
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This is convenient for His purification. However, keep in mind that tags affect protein functionality. It is better to have a cleavable protein tag between this tag and the protein coding sequence. See for example [https://parts.igem.org/Part:BBa_K1319001 BBa_K1319001]
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===Source===
 
===Source===

Revision as of 11:51, 30 October 2017


6xHis/Twin strep


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is convenient for His purification. However, keep in mind that tags affect protein functionality. It is better to have a cleavable protein tag between this tag and the protein coding sequence. See for example BBa_K1319001

Source

synthetic

References