Difference between revisions of "Part:BBa K2323010:Design"
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===Source=== | ===Source=== | ||
| − | Leptotrichia wadei. | + | Leptotrichia wadei. |
===References=== | ===References=== | ||
Sequence was taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017) | Sequence was taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017) | ||
Latest revision as of 11:49, 30 October 2017
Lwa Cas13a coding sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1286
Illegal BglII site found at 2966 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2365
Design Notes
This is a large protein, and the coding sequence has to be codon-optimized, or expressed with a strain that is enriched in tRNAs for rare codons (such as E.coli Rosetta2). Furthermore, this protein is not very soluble and should be tagged with a solubility tag, such as SUMO or MBP. N-terminal context affects the activity of the protein.
Source
Leptotrichia wadei.
References
Sequence was taken from J. S. Gootenberg et al., Science 10.1126/science.aam9321 (2017)