Difference between revisions of "Part:BBa J54033:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
As prefix
+
As prefix of Tokyo Standard.
  
 +
*[[Tokyo Standard assembly]]
 +
Tokyo Standard consists of the chain of restriction sites :
 +
SalI-BamHI-part-BglII-MluI
  
 +
 +
BamHI sequence is "'''G^GATCC'''", and BglII sequence is "'''A^GATCT'''".
 +
 +
They have the sequence "'''GATC'''" in common, so you can assemble parts just like Biobrick assembly.
  
 
===Source===
 
===Source===

Revision as of 16:42, 25 October 2007


PrefixII_SalI,BamHI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As prefix of Tokyo Standard.

Tokyo Standard consists of the chain of restriction sites : SalI-BamHI-part-BglII-MluI


BamHI sequence is "G^GATCC", and BglII sequence is "A^GATCT".

They have the sequence "GATC" in common, so you can assemble parts just like Biobrick assembly.

Source

DNA synthesis

References