Difference between revisions of "Part:BBa K2371016"

Latest revision as of 11:23, 30 October 2017

C-Fluc-dCas9

dCas9 is a catalytically dead Cas9 protein that can combine with target sequence with the help of guide RNA(sgRNA). Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. BGIC-Union split the luciferase into two separate parts(NFluc:BBa_K1689010 and CFluc:BBa_K1689009) and connect them to dCas9 protein via a linker. After connection with sgRNA and reconstitution of proteins, this paired report system can emit luminescence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1689
Illegal NheI site found at 4876
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 30
Illegal BamHI site found at 3968
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 353
Illegal AgeI site found at 492
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2371016 short</partinfo>
-dCas9 is a catalytically dead Cas9 protein that can combine with target sequence with the help of guide RNA(sgRNA). Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. BGIC-Union split the luciferase into two separate parts(NFluc:BBa_2371XXX and CFluc:BBa_2371XXX) and connect them to dCas9 protein via a linker. After connection with sgRNA and reconstitution of proteins, this paired report system can emit luminescence.
+dCas9 is a catalytically dead Cas9 protein that can combine with target sequence with the help of guide RNA(sgRNA). Luciferase is a generic term for the class of oxidative enzymes that produce bioluminescence, and is usually distinguished from a photoprotein. BGIC-Union split the luciferase into two separate parts(NFluc:BBa_K1689010 and CFluc:BBa_K1689009) and connect them to dCas9 protein via a linker. After connection with sgRNA and reconstitution of proteins, this paired report system can emit luminescence.
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