Difference between revisions of "Part:BBa K2276007"
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<partinfo>BBa_K2276007 short</partinfo>
 
<partinfo>BBa_K2276007 short</partinfo>
  
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The part was designed based on the Part:BBa_I13521,while the RBS binding site is modified. The RBS is special designed for strainS whose last 9 nucleotides of the 16S rRNA is ACCTCCTTA.And it can be regard as a improvement for it has a high translation rate compared with Part:BBa_I13521.The promoter is TetR repressible promoter which is also used in the repressilator system. And the mRFP served as the reporter to help us measure the translation rate of the designed RBS.
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The part was designed based on the Part: BBa_I13521, while the RBS binding site is modified. The RBS is special designed for strainS whose last 9 nucleotides of the 16S rRNA are ACCTCCTTA. And it can be regard as a improvement for it has a high translation rate compared with Part: BBa_I13521.The promoter is TetR repressible promoter which is also used in the repressilator system. And the mRFP served as the reporter to help us measure the translation rate of the designed RBS.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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We constructed strains that contain Part:BBa K2276007 and measure the fluorescence intensity and OD600 changes of it.Due to the limit of the expriment condition, we measure the change at the first 8hours and 10~16 hours after inoculated separately.Here is the result.
 
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[[File:SCU-China 2017 RBS fluorescence intensity over time .png|420px|thumb|right]]
 
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Figure 1 The fluorescence intensity changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.
 
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[[File:SCU-China 2017 RBS fluorescence intensity over time.png|420px|thumb|right]]
 
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Figure 2 The OD600 changes over time(0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.
 
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    We can see that Part:BBa K2276007 has a much strong expression than Part:BBa_I13521.
 
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2276007 parameters</partinfo>
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<partinfo> BBa_K2276007 parameters</partinfo>
 
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===Results===
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We constructed strains that contain Part:BBa K2276007 and measure the fluorescence intensity and OD600 changes of it. Due to the limit of the experiment condition, we measure the change at the first 8hours and 10~16 hours after inoculated separately. Here is the result. We can see that Part: BBa_K2276007 has a much strong expression than Part: BBa_I13521.
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The fluorescence intensity changes over time (0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. TetR represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.
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The OD600 changes over time (0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. TetR represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.
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===Source===
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BBa_E2050
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===References===
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[1] Shaner et al, 2004. Nat Biotech (22):1567-1571

Revision as of 11:11, 30 October 2017

a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP

The part was designed based on the Part: BBa_I13521, while the RBS binding site is modified. The RBS is special designed for strainS whose last 9 nucleotides of the 16S rRNA are ACCTCCTTA. And it can be regard as a improvement for it has a high translation rate compared with Part: BBa_I13521.The promoter is TetR repressible promoter which is also used in the repressilator system. And the mRFP served as the reporter to help us measure the translation rate of the designed RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 634
    Illegal AgeI site found at 746
  • 1000
    COMPATIBLE WITH RFC[1000]


Results

We constructed strains that contain Part:BBa K2276007 and measure the fluorescence intensity and OD600 changes of it. Due to the limit of the experiment condition, we measure the change at the first 8hours and 10~16 hours after inoculated separately. Here is the result. We can see that Part: BBa_K2276007 has a much strong expression than Part: BBa_I13521.

The fluorescence intensity changes over time (0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. TetR represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

The OD600 changes over time (0h~16h). P1 represent the strain containing part: BBa_K2276007. P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. TetR represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

Source

BBa_E2050

References

[1] Shaner et al, 2004. Nat Biotech (22):1567-1571