Difference between revisions of "Part:BBa K2247001"

(→‎Characterization)
(→‎Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.)
Line 21: Line 21:
 
=====Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.=====
 
=====Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.=====
  
−
This figure shows another proof of the Gal4-inducible expression from the pGalS promoter (BBa_K2247000). The above mentioned yeast strain JDYY001 was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, also validating the function of pGalS promoter.
+
This figure shows the Gal4-inducible expression of GFP from the pGalS promoter (BBa_K2247000). The yeast strain JDYY001 (endogenous Gal4 and Gal80 knockout) was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, validating the function of pGalS promoter.
  
−
 
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->

Revision as of 10:33, 30 October 2017


pGalS-GFP

This part encodes a GFP gene (BBa_K895006) under the pGalS yeast promoter, used to test the Gal4-dependent inducible expression from pGalS promoter (for detail, see BBa_K2247000).


Usage and Biology

See BBa_K2247000.

This part is mainly used to test the function of the yeast pGalS promoter (BBa_K2247000). The expression of GFP can be induced by galactose, or yeast Gal4 protein.


Characterization

PGalS-GFP AD-BD.jpeg
PGalS-GFP AD-BD-2.jpeg


Figure 1. Yeast GFP expression under the pGalS promoter upon Gal4 induction.

This figure shows the Gal4-inducible expression of GFP from the pGalS promoter (BBa_K2247000). The yeast strain JDYY001 (endogenous Gal4 and Gal80 knockout) was transformed with plasmids encoding pGalS-GFP and Gal4. These representative images showed successful expression of GFP under pGalS induced by Gal4 protein, validating the function of pGalS promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 453
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 54
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1130