Difference between revisions of "Part:BBa I13521"

Revision as of 10:23, 30 October 2017

Ptet mRFP

Constitutive on mRFP (positive control)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 635
Illegal AgeI site found at 747
1000
COMPATIBLE WITH RFC[1000]

Pictures

BBa_I13521 visualized under a non-UV lightbox
BBa_I13521 visualized under 254nm wavelength UV lightbox

The followings were edited by iGEM17_SCU-China

Improvement

We altered the RBS region and constructed new parts that has higher translation rate. To test the performance, we measure the fluorescence intensity and OD600 changes of the bacteria liquid over time within 16hours after inoculated. The result shows that we indeed improve the Part:BBa_I13521 and got a stronger RBS. Fluorescence quenching may explain the drop of the fluorescence intensity of P3 strain at 16h because the concentration is too high

Fig 1.The fluorescence intensity changes over time (0h~16h).P1 represent the strain containing part: BBa_K2276007.P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

Fig 2.The OD600 changes over time (0h~16h).P1 represent the strain containing part: BBa_K2276007.P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence. B0034 represent the strain that containing Part: BBa_I13521.

@@ Line 27: / Line 27: @@
 To test the performance, we measure the fluorescence intensity and OD600 changes of the bacteria liquid over time within 16hours after inoculated.
 The result shows that we indeed improve the Part:BBa_I13521 and got a stronger RBS.
+Fluorescence quenching may explain the drop of the fluorescence intensity of P3 strain at 16h because the concentration is too high
 [[File:FT3.png|600px|thumb|center|'''Fig 1'''.The fluorescence intensity changes over time (0h~16h).P1 represent the strain containing part: BBa_K2276007.P2 represent the strain containing part: BBa_K2276008. P3 represent the strain containing part: BBa_K2276010. Tet R represent the strain that only has tetR repressible promoter without RBS and protein coding sequence.