Difference between revisions of "Part:BBa K2276007"
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | We constructed strains that contain Part:BBa K2276007 and measure the fluorescence intensity and OD600 changes of it.Due to the limit of the expriment condition, we measure the change at the first 8hours and 10~16 hours after inoculated separately.Here is the result. | |
[[File:SCU-China 2017 RBS fluorescence intensity over time .png|420px|thumb|right]] | [[File:SCU-China 2017 RBS fluorescence intensity over time .png|420px|thumb|right]] | ||
Revision as of 09:08, 30 October 2017
a composite of TetR repressible promoter、special designed RBS and the coding sequence of mRFP
The part was designed based on the Part:BBa_I13521,while the RBS binding site is modified. The RBS is special designed for strainS whose last 9 nucleotides of the 16S rRNA is ACCTCCTTA.And it can be regard as a improvement for it has a high translation rate compared with Part:BBa_I13521.The promoter is TetR repressible promoter which is also used in the repressilator system. And the mRFP served as the reporter to help us measure the translation rate of the designed RBS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 634
Illegal AgeI site found at 746 - 1000COMPATIBLE WITH RFC[1000]