Difference between revisions of "Part:BBa K876070:Experience"
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We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium.
 
We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium.
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[[Image: BIT Figure OD copper 25L.png|thumb|center|500px|]]
  
 
===User Reviews===
 
===User Reviews===

Revision as of 08:32, 30 October 2017


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Applications of BBa_K876070

Group:iGEM_BIT2017

Author:WENJIA WANG

We transformed the part BBa_K876070 into the E.coli ΔLysA, and cultured in LB medium overnight. Then aliquots of 100ul were transferred into M9 medium to deplete lysine. After 10h, aliquots of 100ul were transferred into M9 medium with lysine. Lysine was added in increments of 0.5ug/mL to establish a range of 0-3ug/mL. OD600 was measured with microplate reader every hour. The result shows that the OD600 and GFP fluorescence grows obviously with the concentration gradient of the lysine. But its growth rate and GFP fluorescence is much lower than that in LB medium.

BIT Figure OD copper 25L.png

User Reviews

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