Difference between revisions of "Part:BBa K2440007"

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RNF8 3-UTR

This is the 3’prime untranslated region of RNF8 gene. It is the section of mRNA that immediately follows the translation termination codon and contains regulatory regions that post-transcriptionally influence gene expression. ¹ .

Usage and Biology

RNF8 gene is a human gene which encodes an enzyme called E3 ubiquitin-protein ligase RNF8. ²

RNF8 plays an important role in the immune system and in the DNA repair process. ³

The RNF8 protein contains a RING finger motif and an FHA domain. It could interact with several class II ubiquitin-conjugating enzymes and participate in the ubiquitination reaction of certain nuclear proteins. ⁴

There are 3 DNA repair pathways by which RNF8 promotes the repairing of DNA damage: homologous recombinational repair (HRR), non-homologous end joining (NHEJ), and nucleotide excision repair (NER). ^4-6

The double-strand break in DNA could be repaired by either HRR and NHEJ. While the pyrimidine dimers, which are induced by UV, could be repaired by NER. ^5-7

The depletion of RNF8 protein causes cell growth inhibition and cell cycle arrest. ⁸

Sequence and Features

Sequence and Features