Difference between revisions of "Part:BBa K2440007"

 
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<partinfo>BBa_K2440007 short</partinfo>
 
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This is the 3’prime untranslated region of RNF8 gene. It is the section of mRNA that immediately follows the translation termination codon and contains regulatory regions that post-transcriptionally influence gene expression. <sup>1 </sup>.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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RNF8 gene is a human gene which encodes an enzyme called E3 ubiquitin-protein ligase RNF8. <sup>2 </sup>
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RNF8 plays an important role in the immune system and in the DNA repair process. <sup>3 </sup>
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The RNF8 protein contains a RING finger motif and an FHA domain. It could interact with several class II ubiquitin-conjugating enzymes and participate in the ubiquitination reaction of certain nuclear proteins. <sup>4</sup>
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There are 3 DNA repair pathways by which RNF8 promotes the repairing of DNA damage: homologous recombinational repair (HRR), non-homologous end joining (NHEJ), and nucleotide excision repair (NER). <sup>456 </sup>
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The double-strand break in DNA could be repaired by either HRR and NHEJ. While the pyrimidine dimers, which are induced by UV, could be repaired by NER. <sup>567 </sup>
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The depletion of RNF8 protein causes cell growth inhibition and cell cycle arrest. <sup>8 </sup>
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===Sequence and Features===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 07:01, 30 October 2017


RNF8 3-UTR

This is the 3’prime untranslated region of RNF8 gene. It is the section of mRNA that immediately follows the translation termination codon and contains regulatory regions that post-transcriptionally influence gene expression. 1 .

Usage and Biology

RNF8 gene is a human gene which encodes an enzyme called E3 ubiquitin-protein ligase RNF8. 2

RNF8 plays an important role in the immune system and in the DNA repair process. 3

The RNF8 protein contains a RING finger motif and an FHA domain. It could interact with several class II ubiquitin-conjugating enzymes and participate in the ubiquitination reaction of certain nuclear proteins. 4

There are 3 DNA repair pathways by which RNF8 promotes the repairing of DNA damage: homologous recombinational repair (HRR), non-homologous end joining (NHEJ), and nucleotide excision repair (NER). 456

The double-strand break in DNA could be repaired by either HRR and NHEJ. While the pyrimidine dimers, which are induced by UV, could be repaired by NER. 567

The depletion of RNF8 protein causes cell growth inhibition and cell cycle arrest. 8

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1435
    Illegal XbaI site found at 3
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1435
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1435
    Illegal BglII site found at 927
    Illegal BglII site found at 1755
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1435
    Illegal XbaI site found at 3
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1435
    Illegal XbaI site found at 3
    Illegal PstI site found at 637
    Illegal PstI site found at 2007
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1116