Difference between revisions of "Part:BBa K2374001:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We get this 452bp sequence from FlyBase ([http://flybase.org/reports/FBgn0005626.html] Dmel\ple)
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 +
 +
 
site direct mutangenesis:
 
site direct mutangenesis:
 
EcoR I (184)  GAATTC->GATTTC
 
EcoR I (184)  GAATTC->GATTTC
 
Xba I  (219)  TCTAGA->TGTAGA
 
Xba I  (219)  TCTAGA->TGTAGA
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−
 
  
 
===Source===
 
===Source===

Revision as of 02:52, 30 October 2017


TH (ple) promoter-> (fruit fly)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 137
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We get this 452bp sequence from FlyBase ([http://flybase.org/reports/FBgn0005626.html] Dmel\ple)


site direct mutangenesis: EcoR I (184) GAATTC->GATTTC Xba I (219) TCTAGA->TGTAGA

Source

NT_037436.4 (NCBI)

References