Difference between revisions of "Part:BBa K2300002"

Revision as of 02:27, 30 October 2017

HydG (2017)

The original HydG (part number BBa_K1998010) was not submitted in 2016 as the DNA sequence had a mutation present. HydG was re-synthesised by Macquarie iGEM 2017. HydG is a radical S-adenosyl methionine (SAM) enzyme which functions as part of the [FeFe] hydrogenase maturation enzyme complex. All organisms with [FeFe] hydrogenase contain homologues of this gene.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Unknown
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1327
1000
COMPATIBLE WITH RFC[1000]

Biology & Literature

Part Verification

Protein information

HydG
Mass: 63.74 kDa
Sequence:
MSVPLQCNAGRLLAGQRPCGVRARLNRRVCVPVTAHGKASATREYAGDFLPGTTISHAWSVERETHHRYRNPAEWINEAA IHKALETSKADAQDAGRVREILAKAKEKAFVTEHAPVNAESKSEFVQGLTLEECATLINVDSNNVELMNEIFDTALAIKE RIYGNRVVLFAPLYIANHCMNTCTYCAFRSANKGMERSILTDDDLREEVAALQRQGHRRILALTGEHPKYTFDNFLHAVN VIASVKTEPEGSIRRINVEIPPLSVSDMRRLKNTDSVGTFVLFQETYHRDTFKVMHPSGPKSDFDFRVLTQDRAMRAGLD DVGIGALFGLYDYRYEVCAMLMHSEHLEREYNAGPHTISVPRMRPADGSELSIAPPYPVNDADFMKLVAVLRIAVPYTGM ILSTRESPEMRSALLKCGMSQMSAGSRTDVGAYHKDHTLSTEANLSKLAGQFTLQDERPTNEIVKWLMEEGYVPSWCTAC YRQGRTGEDFMNICKAGDIHDFCHPNSLLTLQEYLMDYADPDLRKKGEQVIAREMGPDASEPLSAQSRKRLERKMKQVLEGEHDVYL

References

Posewitz, M.C., King, P.W., Smolinski, S.L., Zhang, L., Seibert, M. and Ghirardi, M.L., 2004. Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase. Journal of Biological Chemistry, 279(24), pp.25711-25720. Vancouver

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2300002 short</partinfo>
-The original HydG part number BBa_K1998010 had a problem in the sequence so it was resynthesized and was not submitted last year.
+The original HydG (part number BBa_K1998010) was not submitted in 2016 as the DNA sequence had a mutation present. HydG was re-synthesised by Macquarie iGEM 2017. HydG is a radical S-adenosyl methionine (SAM) enzyme which functions as part of the [FeFe] hydrogenase maturation enzyme complex. All organisms with [FeFe] hydrogenase contain homologues of this gene.
 <!-- Add more about the biology of this part here