Difference between revisions of "Part:BBa K2374003:Design"

Revision as of 00:42, 30 October 2017

ple (Tyrosine 3-monooxygenase, TH) -> (fruit fly)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1157
Illegal XhoI site found at 289
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 535
Illegal BsaI site found at 1501
Illegal BsaI.rc site found at 253
Illegal BsaI.rc site found at 1019
Illegal BsaI.rc site found at 1168

Design Notes

site direct mutangenesis: Pst I (647) CTGCAG->CTGCTG

According to our experiment to judge， the ple coding sequence is hard to clone from Drosophila's cDNA library because of its multi-segment repeats. So we recommend that you obtain directly from the constructed plasmid, or synthesize it directly.

               <img src="" alt="1% gel electrophoresis of plasmid" style="width:100%">

                 pUAST-pleP-Gal80ts

Source

Drosophila melanogaster chromosome 3L [NT_037436.4] (NCBI)

References

JANICE A. FISCHER, et al. GAL4 activates transcription in Drosophila. Nature 332, 853 - 856 (1988)

@@ Line 13: / Line 13: @@
 [[File:2017tongji image registry UASTHtest.png|center|200px|标题]]
+              <div class="demo-card-wide mdl-card mdl-shadow--2dp" style="width:30%; float:left; margin:0%; margin-right:5%">
+                <img src="https://static.igem.org/mediawiki/2017/b/b8/2017tongji_wiki_image_process_13.png" alt="1% gel electrophoresis of plasmid" style="width:100%">
+                <div class="mdl-card__supporting-text" style="font-size: 70%; position: absolute; bottom:0px; right:0px; color:#f0f0f0; text-align:right">
+                  <!-- white:#f0f0f0 or black:#0F0F0F -->
+                  pUAST-pleP-Gal80ts
+                </div>
+              </div>
 ===Source===