Difference between revisions of "Part:BBa K2271066"

Revision as of 23:52, 29 October 2017

mRuby-ePTS1

Usage and Biology

The part contains a mRuby fluorezenz protein with an enhanced pts1 sequence, described by Dueber et. Al, with a TDH3 Promotor and a HHF1 Terminator. mRuby is a mutant oft he fluorezent protein gfp with excitation and emission wavelengths at 559 nm/600nm . With the C-terminal enhanced pts1 (peroxisomal targeting sequnce) the mRuby is imported into the peroxisome. The part can be used as a peroxisomal matrix marker in S. cerevisiea.

Experimental Design and Results

Figure 1mRuby fused with PTS1 localised peroxisomal typical in the cells

d

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 860
Illegal BamHI site found at 1577
Illegal XhoI site found at 1613
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2271066 short</partinfo>
+===Usage and Biology===
 The part contains a mRuby fluorezenz protein with an enhanced pts1 sequence, described by Dueber et. Al, with a TDH3 Promotor and a HHF1 Terminator. mRuby is a mutant oft he fluorezent protein gfp with excitation and emission wavelengths at 559 nm/600nm  . With the C-terminal enhanced pts1 (peroxisomal targeting sequnce) the mRuby is imported into the peroxisome. The part can be used as a peroxisomal matrix marker in <i>S. cerevisiea.</i>
+=== Experimental Design and Results===
+[[File:Igemducgn2017mRuby-PTS1.png|200px|thumb|right|'''Figure 1'''mRuby fused with PTS1 localised peroxisomal typical in the cells]]
+<p>d</p>
 <!-- Add more about the biology of this part here