Difference between revisions of "Part:BBa K2289001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This is a noncoding ssDNA sequence, for use you have to order directly | + | This is a noncoding functional ssDNA sequence, for use you have to order directly as an oligo. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | The DNAzyme sequence is on the Registry Part:BBa_K1614007 and it was submited by team Heidelberg 2015. Aptamer sequence, the STX M30f, was selected by a pool of aptarmers described in Zheng et. al., 2015. And the linkers where selected by the JAWS software developed by team Heidelberg, for this special case we selected de J_2 linkers. | + | The DNAzyme sequence is on the Registry Part:BBa_K1614007 (https://parts.igem.org/Part:BBa_K1614007) and it was submited by team Heidelberg 2015. Aptamer sequence, the STX M30f (Part:BBa_K2289004), was selected by a pool of aptarmers described in Zheng et. al., 2015 (https://parts.igem.org/Part:BBa_K2289004). And the linkers where selected by the JAWS software developed by team Heidelberg, for this special case we selected de J_2 linkers. |
===References=== | ===References=== | ||
| + | X. Zheng, B. Hu, SX. Gao, D.J. Liu, M.J. Sun, B.H. Jiao, L.H. Wang. (2015). A saxitoxin-binding aptamer whith higher affinity and inhibitory activity optimized by rational site-directed mutagenesis and truncation. Toxicon 101. 41-47. | ||
Latest revision as of 22:39, 29 October 2017
STX Aptazyme J_2
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a noncoding functional ssDNA sequence, for use you have to order directly as an oligo.
Source
The DNAzyme sequence is on the Registry Part:BBa_K1614007 (https://parts.igem.org/Part:BBa_K1614007) and it was submited by team Heidelberg 2015. Aptamer sequence, the STX M30f (Part:BBa_K2289004), was selected by a pool of aptarmers described in Zheng et. al., 2015 (https://parts.igem.org/Part:BBa_K2289004). And the linkers where selected by the JAWS software developed by team Heidelberg, for this special case we selected de J_2 linkers.
References
X. Zheng, B. Hu, SX. Gao, D.J. Liu, M.J. Sun, B.H. Jiao, L.H. Wang. (2015). A saxitoxin-binding aptamer whith higher affinity and inhibitory activity optimized by rational site-directed mutagenesis and truncation. Toxicon 101. 41-47.