Difference between revisions of "Part:BBa K2323013:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
There's no special considerations here, other than the RBS needs to be correctly transcribed, and accessible for the ribosome. It should therefore be a few nucleotides after the first transcribed nucleotide of the mRNA
+
There's no special considerations here. It's a spacer sequence. It should be a multiple of 3 nucleotides, to not shift the reading frame.
  
  

Latest revision as of 20:48, 29 October 2017


Spacer between His tag and coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There's no special considerations here. It's a spacer sequence. It should be a multiple of 3 nucleotides, to not shift the reading frame.


Source

random DNA sequence

References